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ELISAԇELISAԇСELISAԇСELISAԇԇwѪ塢M(jn)ژ(bio)(zhn)Ʒ΢B(yng)
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|(zh)ٵø1TIMP-1ELISAԇжX

ţ|(zh)ٵø1TIMP-1ELISAԇжX

r(ji)h

ُȣ156    ԭa(chn)أ

l(f)r(sh)g2018/8/10 9:30:48

|(zh)ٵø1TIMP-1ELISAԇжXwⶨzyѪ塢Ѫ{M(x)弰P(gun)Һwӱ|(zh)ٵø1TIMP-1ĺ

Ԕ(x)Ϣ
 a(chn)Ʒ
|(zh)ٵø1TIMP-1elisaԇX
z(bio)ѪѪ{ҺظˮXҺ(x)B(yng)
Ҏ(gu) 96T
Һw
棺2-8
Ҫδṩԇ
1. (bio)(zhn)Ҏ(gu)ø(bio)x
2. xęC(j)
3. 늟B(yng)
4. ɃԇܺEppendof
5. ϵп{(dio)(ji)Һ^һΙzyƷ^r(sh)öͨҺ
6. sˮƿ

|(zh)ٵø1TIMP-1ELISAԇжXE
(sh)(yn)_ʼǰԇ(yng)ƽҜ(ԇֱ37ܽ)ԇƷϡጕr(sh)򣬻r(sh)M(sh)(yn)ǰ(yng)A(y)yƷƷ^ߕr(sh)(yng)ƷM(jn)ϡʹϡጺĘƷԇеęzyӋ(j)r(sh)ٳ(yng)ϡጱ(sh)
1.ӘӣքeO(sh)հ׿ס(bio)(zhn)yƷհ׿׼ӘƷϡҺ100μl׷քeӘ(bio)(zhn)ƷyƷ100μlעⲻҪКӘӌƷø(bio)׵ײM|ױpp΄ӻø(bio)wĤ37淴(yng)120鱣C(sh)(yn)Y(ji)ЧÿΌ(sh)(yn)ՈʹµĘ(bio)(zhn)ƷҺ
2.ȥҺw˦ϴÿ׼әzyҺAҺ 100μl(ʹǰһСr(sh)(ni))ø(bio)ϸĤ, 37淴(yng)60
3.60犺ȥ׃(ni)Һw˦ɣϴ3ÿν1-2s400μl/ÿ˦(ҲpČ׃(ni)Һwĸ)
4.ÿ׼әzyҺBҺ(ͬzyAҺ) 100μlø(bio)ϸĤ37淴(yng)60犡
5.60犺ȥ׃(ni)Һw˦ϴ5ÿν1-2350μl/ÿ˦(ҲpČ׃(ni)Һwĸ)
6.ÿ׼ӵҺ90μlø(bio)ϸĤ37ܹ@ɫ(30犃(ni)˕r(sh)ۿҊ(bio)(zhn)Ʒǰ3-4@ݶmɫ3-4ݶȲ@ɽKֹ)
7.ÿ׼ӽKֹҺ50μlKֹ(yng)˕r(sh){(ln)ɫD(zhun)SɫKֹҺļ(yng)McҺļͬ˱C(sh)(yn)Y(ji)Ĝ(zhn)_ԣﷴ(yng)r(sh)g(yng)MKֹҺ
8.ø“(lin)x450nmLy׵Ĺܶ(ODֵ) ڼӽKֹҺM(jn)Йzy

ע(xing)
|(zh)ٵø1TIMP-1ELISAԇжX(sh)(yn)ע(xing)
1.ԇ(zhn)䣺ԇʹǰ_(d)ҜأʹúՈfҪ󱣴ԇ(sh)(yn)ՈʹһԵ^⽻Ⱦ
2.ӘӣӘӻԇr(sh)Ոעȡ(bio) / (bio)(zhn)ƷøY(ji)r(sh)(g)c*һ(g)׼Ә֮gĕr(sh)gg̫󣬌(do)²ͬ “A(y)"r(sh)gĶ@Ӱ푵yֵĜ(zhn)_Լ؏(f)һμӘӕr(sh)g((bio)(zhn)ƷИƷ)10犃(ni)(bio)(sh)]ʹöҺӘ
3.ֹƷl(f)ԇ(yn)r(sh)(yng)Н񲼵]Ѓ(ni)ø(bio)wĤԱҺwl(f)ϴ(yng)MM(jn)²κΕr(sh)(yng)ø(bio)̎ڸB(ti);ͬr(sh)(yng)(yn)ؽoķr(sh)g͜ض
4.ϴ죺ϴ^з(yng)КϴҺ(yng)ڞVϳĸVֱӷ뷴(yng)ˮͬr(sh)ҪךҺwָӡӰ*ø(bio)xx(sh)
5.ԇƣDetection ADetection BʹǰՈ˦»ٕr(sh)x̎ʹܱڻƿwҺweܵ(bio)(zhn)ƷzyҺAҺzyҺBҺՈ(j)ʹʹ(yng)ϡҺܻՈ_Ø(bio)(zhn)ƷҺMҪ΢(ȡzyҺAr(sh)һβҪС10μl)Աڲ(zhn)_ϡጶɵĝ`Ո؏(f)ʹϡ^Ę(bio)(zhn)ƷzyҺAҺzyҺBҺ
6.(yng)r(sh)gĿƣՈr(sh)^췴(yng)׵ɫ׃(ÿ10)ɫ^Ո?zh)ǰKֹҺKֹ(yng)ⷴ(yng)^(qing)ĶӰø(bio)xܶx(sh)
7.ՈܹⱣڃ͜r(sh)⏊(qing)ֱhzyƷr(sh)O(sh)pלyԱCzyY(ji)Ĝ(zhn)_
(bio)дy|(zh)^ՈϡጺٜyӋ(j)r(sh)Ո*ϡጱ(sh)
˾Ҋa(chn)Ʒ
˳ҊELISAԇϵ
ˣIL-1IL-1αIL-1βIL-2IL-2RIL-27IL-23IL-17IL-18IL-9IL-8IL-6IL-5IL-4IL-2IL-13IL-11IL-10IL-12P70IL-12P40IFN-ωIFN-βIFN-α IFN-γTNF-βTNF-αsICAM-1LEPMMP-3
ҊELISAԇϵ
TNF-αIL-1βIL-7IL-32IL-21IL-27IL-23IFN-γTGF-αTGF-β1
СҊELISAԇϵ
СIFN-γIL-1αIL-1βIL-13IL-10IL-11IL-17TNF-αTNF-βVEGFTGF-β1
iuֲȶNNELISAԇУԇн(jng)^(yn)CCЧ؏(f)Լѡ

•r(sh)g2024/11/25 9:13:04

ھ

ھԃ𾴵Ŀ͑ã҂M؏(f)ԃ

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2.Ո挍(sh)ķӳa(chn)Ʒr,Ҫ졢_{猦a(chn)ƷκɆ,Ҳԃ

3.δ(jng)վͬ⣬κ˲ñԲl(f)(g)˻F(tun)wľЏV|(zh)ϢՓ

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