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ELISAԇELISAԇСELISAԇСELISAԇԇwѪM(jn)ژ(bio)(zhn)Ʒ΢B(yng)
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BIM͸ײIgGwHAV IgGELISAԇ

BIM͸ײIgGwHAV IgGELISAԇ

rh

ُȣ174    ԭa(chn)أ

l(f)rg2018/8/10 9:50:05

BIMţ͸ײIgGwHAV IgGELISAԇwⶨzyѪѪ{M(x)弰P(gun)Һwӱţ͸ײIgGwHAV IgGĺ

(bio)BIM͸ײIgGwHAV   IgGELISAԇ   

Ԕ(x)Ϣ
 a(chn)Ʒ
BIMţ͸ײIgGwHAV IgGelisaԇ
z(bio)ѪѪ{ҺظˮXҺ(x)B(yng)
Ҏ(gu) 96T
Һw
棺2-8
Ҫδṩԇ
1. (bio)(zhn)Ҏ(gu)ø(bio)x
2. xęC(j)
3. 늟B(yng)
4. ɃԇܺEppendof
5. ϵп{(dio)(ji)Һ^һΙzyƷ^röͨҺ
6. sˮƿ

BIM͸ײIgGwHAV IgGELISAԇE
(sh)(yn)_ʼǰԇ(yng)ƽҜ(ԇֱ37ܽ)ԇƷϡጕrrMݡ(sh)(yn)ǰ(yng)A(y)yƷƷ^ߕr(yng)ƷM(jn)ϡʹϡጺĘƷԇеęzyӋ(j)rٳ(yng)ϡጱ(sh)
1.ӘӣքeO(sh)հ׿(bio)(zhn)סyƷհ׿׼ӘƷϡҺ100μl׷քeӘ(bio)(zhn)ƷyƷ100μlעⲻҪКӘӌƷø(bio)׵ײM|ױpp΄ӻø(bio)wĤ37淴(yng)120鱣C(sh)(yn)Y(ji)ЧԣÿΌ(sh)(yn)ՈʹµĘ(bio)(zhn)ƷҺ
2.ȥҺw˦ɣϴÿ׼әzyҺAҺ 100μl(ʹǰһСr(ni))ø(bio)ϸĤ, 37淴(yng)60
3.60犺ȥ׃(ni)Һw˦ɣϴ3ÿν1-2犣s400μl/ÿ˦(ҲpČ׃(ni)Һwĸ)
4.ÿ׼әzyҺBҺ(ͬzyAҺ) 100μlø(bio)ϸĤ37淴(yng)60犡
5.60犺ȥ׃(ni)Һw˦ϴ5Σÿν1-2350μl/ÿ˦(ҲpČ׃(ni)Һwĸ)
6.ÿ׼ӵҺ90μlø(bio)ϸĤ37ܹ@ɫ(30犃(ni)˕rۿҊ(bio)(zhn)Ʒǰ3-4@ݶmɫ3-4ݶȲ@ɽKֹ)
7.ÿ׼ӽKֹҺ50μlKֹ(yng)˕r{(ln)ɫD(zhun)SɫKֹҺļ(yng)McҺļͬ˱C(sh)(yn)Y(ji)Ĝ(zhn)_ԣﷴ(yng)rg(yng)MKֹҺ
8.ø“(lin)x450nmLy׵Ĺܶ(ODֵ) ڼӽKֹҺM(jn)Йzy

ע(xing)
BIM͸ײIgGwHAV IgGELISAԇ(sh)(yn)ע(xing)
1.ԇ(zhn)䣺ԇʹǰ_(d)ҜأʹúՈfҪ󱣴ԇ(sh)(yn)ՈʹһԵ^⽻Ⱦ
2.ӘӣӘӻԇrՈעȡ(bio) / (bio)(zhn)ƷøY(ji)rc*һ׼Ә֮gĕrgg̫󣬌(do)²ͬ “A(y)"rgĶ@Ӱ푵yֵĜ(zhn)_Լ؏(f)һμӘӕrg((bio)(zhn)ƷИƷ)10犃(ni)(bio)(sh)࣬]ʹöҺӘ
3.ֹƷl(f)ԇ(yn)r(yng)Н񲼵]Ѓ(ni)ø(bio)wĤԱҺwl(f)ϴ(yng)MM(jn)²κΕr(yng)ø(bio)̎ڸB(ti);ͬr(yng)(yn)ؽoķrg͜ض
4.ϴ죺ϴ^з(yng)КϴҺ(yng)ڞVϳĸVֱӷ뷴(yng)ˮͬrҪךҺwָӡӰ*ø(bio)xx(sh)
5.ԇƣDetection ADetection BʹǰՈ˦»ٕrx̎ʹܱڻƿwҺweܵ(bio)(zhn)ƷzyҺAҺzyҺBҺՈ(j)ʹʹ(yng)ϡҺܻՈ_Ø(bio)(zhn)ƷҺMҪ΢(ȡzyҺArһβҪС10μl)Աڲ(zhn)_ϡጶɵĝ`Ո؏(f)ʹϡ^Ę(bio)(zhn)ƷzyҺAҺzyҺBҺ
6.(yng)rgĿƣՈr^췴(yng)׵ɫ׃(磬ÿ10)ɫ^Ո?zh)ǰKֹҺKֹ(yng)ⷴ(yng)^(qing)ĶӰø(bio)xܶx(sh)
7.ՈܹⱣ棬ڃ͜r⏊(qing)ֱhzyƷrO(sh)pלyԱCzyY(ji)Ĝ(zhn)_ԡ
(bio)дy|(zh)^ՈϡጺٜyӋ(j)rՈ*ϡጱ(sh)
˾Ҋa(chn)Ʒ
˳ҊELISAԇϵ
ˣIL-1IL-1αIL-1βIL-2IL-2RIL-27IL-23IL-17IL-18IL-9IL-8IL-6IL-5IL-4IL-2IL-13IL-11IL-10IL-12P70IL-12P40IFN-ωIFN-βIFN-α IFN-γTNF-βTNF-αsICAM-1LEPMMP-3
ҊELISAԇϵ
TNF-αIL-1βIL-7IL-32IL-21IL-27IL-23IFN-γTGF-αTGF-β1
СҊELISAԇϵ
СIFN-γIL-1αIL-1βIL-13IL-10IL-11IL-17TNF-αTNF-βVEGFTGF-β1
iuֲȶNNELISAԇԇн(jng)^(yn)CCЧ؏(f)Լѡ

•rg2024/11/18 9:49:47

ھ

ھԃ𾴵Ŀ͑҂M؏(f)ԃ

dȤĮa(chn)Ʒ* ߀]ݔdȤĮa(chn)Ʒ
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“(lin)ϵ* ߀]ݔ“(lin)ϵ
“(lin)ϵԒ* ߀]ݔ“(lin)ϵԒ
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1.A񹲺͇P(gun)Ҏ(gu)ؾW(wng)ϵГ(dn)һОֱӻgķ؟(z)

2.Ո挍(sh)ķӳa(chn)Ʒr,Ҫ_{猦a(chn)ƷκɆ,Ҳԃ

3.δ(jng)վͬκ˲ñԲl(f)˻F(tun)wľЏV|(zh)ϢՓ

P(gun)

| ˾B| a(chn)Ʒչʾ| ˾„| g(sh)| “(lin)ϵ҂| ͑

xW(wng) O(sh)Ӌ(j)δ(jng)S䛱ؾ. “(lin)ϵˣX(jng)“(lin)ϵԒ13636351217 ICP䰸̖ICP11004148̖-11 L8844227

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