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I(yng)a(chn)Ʒ

ELISAԇELISAԇELISAԇССELISAԇԇwѪM(jn)ژ(bio)(zhn)Ʒ΢B(yng)
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(dng)ǰλãϺh(yun)\g(sh)l(f)չ޹˾ > a(chn)Ʒ > ELISAԇ > M(jn)ELISAԇ > ߲FMDVELISAԇf(shu)

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a(chn)Ʒ(jin)

߲FMDVELISAԇf(shu)

ţ߲FMDVELISAԇf(shu)

r(ji)h

ُ(gu)ȣ165    ԭa(chn)أ

l(f)r(sh)g2018/8/13 16:04:13

߲FMDVELISAԇf(shu)wⶨzy(c)Ѫ塢Ѫ{M(x)弰P(gun)Һwӱ߲FMDVĺ

Ԕ(x)Ϣ
a(chn)ƷQ߲FMDVelisaԇf(shu)
λУ/t(y)Ժ/Ժ
(sh)(yn)ͣAķ/gӷ/(jng)(zhng)
(yng)r(sh)g: 1-5h
ӱwe: 50-100ul
zy(c)L(zhng): 450 nm
(yng)÷wⶨzy(c)Ѫ塢Ѫ{M(x)弰P(gun)Һwӱ
l2-8棻
•a(chn)ƷHЌ(sh)(yn)mR\

a(chn)Ʒ
΢יzy(c)ϵУ׽ԇ,TNFԇ,VEGFԇ,Ipԇ
P(gun)Сәzy(c)ϵУƤ|(zh)ԇ,HCYԇ,T3,T4ԇ
ʳƷȫСәzy(c)ϵУcùELISAzy(c)ԇ,谷ELISAzy(c)ԇ,ĭh(hun)ELISAzy(c)ԇ,SùELISAzy(c)ԇ
wzy(c)ϵУѪowELISAzy(c)ԇ,AQP-Abԇ,HINIԇ
طNיzy(c)ʮ(g)Nٵİ׵,򵰰ELISAzy(c)ԇС
M(fi)y(c)
ֻјӱ^(gu)(li)҂?yu)?ji)ʡr(sh)gY(ji)ԭʼ(sh)(j)(sh)(j)ṩr(sh)g5-7ң


g(sh)ָ(do)
ȫ̼g(sh)ָ(do)ǰĘ(bio)ռʹ^(gu)в׵ĵط(sh)(yn)Y(ji)Ĕ(sh)(j)ȵ߅elisaԇ(sh)(yn)

߲FMDVELISAԇf(shu)͑Ⱥw

ӆُ(gu)̣

gӭĿІλڸN(xing)Ŀc˾_չͬӴεкpAl(f)չͬM(jn)Ї(gu)zy(c)˜I(y)İl(f)չe۽(jng)(yn)

E
1. Ҝƽ20minXȡlʣlԷܷŻ4档
2. O(sh)Ø(bio)(zhn)Ʒ׺͘ӱ(bio)(zhn)Ʒ׸ӲͬȵĘ(bio)(zhn)Ʒ50μL
3. ӱмy(c)ӱ50μLհ׿ײ
4. հ׿⣬(bio)(zhn)Ʒ׺͘ӱÿ׼^(gu)øHRP(bio)ӛęzy(c)w100μL÷Ĥס(yng)37ˮԡ偻60min
5. ȥҺwˮĸɣÿ׼ӝMϴҺ350μLo1min˦ȥϴҺˮĸɣ؏(f)ϴ5ΣҲϴC(j)ϴ壩
6. ÿ׼AB50μL37ܹ15min
ÿ׼KֹҺ50μL15min(ni)450nmL(zhng)̎y(c)׵ODֵ

߲FMDVELISAԇf(shu)
ע(xing)
1.(yn)Ҏ(gu)ĕr(sh)g͜ضM(jn)МԱC(zhn)_Y(ji)ԇʹǰ_(d)Ҝ20-25ʹúرԇ
2.ϴ岻_Ԍ(do)²(zhn)_ĽY(ji)ڼǰ_Mɿ׃(ni)Һw^(gu)вҪ׌΢׸
3.ךҺwָӡtӰODֵ
4.@ɫҺ(yng)ʟo(w)ɫܜ\ɫѽ(jng)׃{(ln)ĵҺʹ
5.ԇ͘(bio)ĽȾe(cu)`Y(ji)
6.ڃ(ch)͜r(sh)⏊(qing)ֱ
7.ƽҜغٴ_ܷԷˮlϡ
8.κη(yng)ԇܽ|Ư܄Ư܄ɢl(f)ď(qing)ҚwκƯ׳ɷֶ(hu)Ɖԇз(yng)ԇԡ
9.ʹ^(gu)ڮa(chn)Ʒ
܂еĘƷ(yng)Ҏ(gu)ij̎Ʒ͙zy(c)bá

•r(sh)g2024/12/23 9:39:54

ھ

ھԃ𾴵Ŀ͑ã҂(hu)M؏(f)ԃ

dȤĮa(chn)Ʒ* ߀](mi)ݔdȤĮa(chn)Ʒ
Ćλ* ߀](mi)ݔĆλ
“(lin)ϵ* ߀](mi)ݔ“(lin)ϵ
“(lin)ϵԒ* ߀](mi)ݔ“(lin)ϵԒ
Ԕ(x)ַ
] ݔ]ʽ_
Ո(qng)ݔ(du)҂Ҋh* ߀](mi)ݔ(du)҂Ҋh
(yn)Ca* ߀](mi)ݔ(yn)Ca

l(f)

ܰʾ

1.A񹲺͇(gu)P(gun)Ҏ(gu)ؾW(wng)ϵ£Г(dn)һОֱӻgķ؟(z)

2.Ո(qng)挍(sh)ķӳa(chn)Ʒr,Ҫ_{猦(du)a(chn)ƷκɆ(wn),Ҳԃ

3.δ(jng)վͬκ˲ñԲl(f)(g)˻F(tun)wľЏV|(zh)ϢՓ

P(gun)

(y)| ˾B| a(chn)Ʒչʾ| ˾„| g(sh)| “(lin)ϵ҂| ͑

xW(wng) O(sh)Ӌ(j)δ(jng)S䛱ؾ. “(lin)ϵˣX(jng)“(lin)ϵԒ13636351217 ICP䰸̖(ho)ICP11004148̖(ho)-11 L(wn)9091214

I(yng)a(chn)ƷELISAԇELISAԇELISAԇСELISAԇСԇwѪM(jn)ژ(bio)(zhn)Ʒ΢B(yng)

һP(gun)ע҂

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